Indirect Elisa Test Procedure. Prepare developing reagent dilutions antibody alkaline phosphatase conjugates. Bovine serum albumin is used to block the other protein binding sites primary sample antibody is added to the plate and incubated with the antigen.
Block all unbound sites to prevent false positive results. Procedure of indirect elisa coat the micro titer plate wells with antigen. Dispense 50 μl antigen solution coating reagent into the wells of an micro titer plate using pipette and tips.
Elisa assay this immunological assay lecture explains about the elisa test procedure and principle behind the elisa assay including direct indirect and sa.
Bovine serum albumin is used to block the other protein binding sites primary sample antibody is added to the plate and incubated with the antigen. Samples with antibodies are added and washed. Enzyme linked secondary antibody are added and washed. Rabbit monoclonal antibody to the wells and incubate the plate at 37 c.
