Indirect Elisa Procedure. Indirect elisa protocol buffers and reagents. This technique will detect hiv within 5 weeks.
In tube 1 prepare a 4 ml solution of secondary reagent at 200 ng ml in pbsn. It can be performed via two processes known as direct or indirect elisa. Indirect elisa detects antibodies from the test sample.
Repeat this transfer and mix for tubes 3 to 5.
In tube 1 prepare a 4 ml solution of secondary reagent at 200 ng ml in pbsn. It can be performed via two processes known as direct or indirect elisa. Indirect elisa procedure antigen is coated onto the wells by passive adsorption and incubation the plate is washed with pbs to remove unbound antigens. The indirect elisa detects the presence of antibody in a sample.
