Indirect Elisa Diagram. For accurate quantitative results always compare signal of unknown samples against those of a standard curve. See direct elisa protocol buffers and reagents.
References 1 the enzyme linked immunosorbent assay elisa bulletin of the world health organization. In direct elisa primary antibodies are labelled whereas in indirect elisa secondary antibodies are labelled. Indirect elisa is a two step elisa which involves two binding process of primary antibody and labeled secondary antibody.
It is a common laboratory technique which is usually used to measure the concentration of antibodies or antigens in blood.
There are three important differences between indirect and direct elisas as shown in figure 4. References 1 the enzyme linked immunosorbent assay elisa bulletin of the world health organization. Elisa is an antigen antibody reaction. The assay uses a solid phase type of enzyme immunoassay eia to detect the presence of a ligand commonly a protein in a liquid sample using antibodies directed against the protein to be measured.
