Direct And Indirect Elisa Test. The indirect elisa method has high sensitivity since more than one labeled secondary antibody can bind the primary antibody. There are presently four types of elisa test such as.
Direct elisa test uses only primary antibodies to detect the antigen while indirect elisa uses both primary and secondary antibodies. It can be performed via two processes known as direct or indirect elisa. This elisa test is much faster and easier as compared to others.
In a direct elisa you are detecting the presence of an antigen and the primary antibody used is the enzyme linked antibody.
Indirect elisa delivers greater flexibility since different primary antibodies can be used with a single labeled secondary antibody. For most assay systems test solutions containing 1 to 100 ng ml of antigen can be accurately measured. A direct elisa involves coating the plates with a mixture containing the target analyte in this case patient serum. In an indirect elisa you are detecting the antibody and the secondary antibody is enzyme linked.
